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Sains Malaysiana 53(12)(2024): 3291-3305
http://doi.org/10.17576/jsm-2024-5312-13
Inhibition of
Quorum Sensing, Biofilm and Motility of Escherichia coli by Staphylococcus warneri MG1_8 Bioactive Compounds
(Perencatan Penderiaan Kuorum, Biofilem dan Motiliti Escherichia
coli oleh Sebatian Bioaktif Staphylococcus warneri MG1_8)
NATTARIKA
CHAICHANA1, YAOWAPA SUKPONDMA2, DECHA SERMWITTAYAWONG3 & NATTHAWAN SERMWITTAYAWONG1,*
1Division of Biological Science, Faculty of
Science, Prince of Songkla University, Hatyai, Songkhla, 90110, Thailand
Received: 4 June
2024/Accepted: 26 October 2024
Abstract
Quorum
sensing (QS) system in bacteria, mediated by a small signaling molecule called an autoinducer (AI), promotes biofilm formation and other
virulence factors that contribute to reducing the susceptibility of
treatment agents. Escherichia coli is considered bacteria contaminated
in the food industry with their biofilm-forming ability which allows them to
adhere on surfaces. In this study, the cell-free supernatant (CFS) extract of
mangrove forest isolated bacteria, Staphylococcus warneri MG1_8, was purified and its possible quorum sensing inhibitor (QSI) metabolites
were identified. Fraction A was separated from S. warneri MG1_8-CFS extract using gel column chromatography and confirmed as a positive
fraction by its ability to inhibit bioluminescence in the biosensor strain Vibrio campbellii ATCC BAA-1119, showing 76% inhibition.
In addition, pyridoxine (PN) was identified as a major compound in the S. warneri MG1_8 CFS extract. The minimum inhibitory
concentration (MIC) of PN against E. coli ATCC 25922 was 50 mg/mL. The PN at 1/4MIC reduced biofilm formation and cell
adherence in E. coli strains, as observed under scanning electron
microscopy (SEM). Moreover, motility and AI-2 signaling also decreased after exposure to PN at sub-MIC levels. Current research
demonstrates that PN can inhibit biofilm formation and motility by interfering
with AI-2 QS, suggesting it could be considered a novel biocontrol agent to
reduce bacterial contamination in food production.
Keywords:
Autoinducer-2; biofilm formation; Escherichia coli; pyridoxine; quorum sensing
inhibitor
Abstrak
Sistem penderiaan kuorum (QS) dalam bakteria yang dimediasi oleh molekul isyarat kecil yang dipanggil autoinduser (AI) menggalakkan pembentukan biofilem dan faktor virulensi lain yang menyumbang kepada pengurangan kerentanan agen rawatan. Escherichia coli dianggap bakteria tercemar dalam industri makanan dengan keupayaan membentuk biofilem yang membolehkan mereka melekat pada permukaan. Dalam kajian ini, ekstrak supernatan bebas sel (CFS) bakteria pencilan hutan bakau, Staphylococcus warneri MG1_8, telah dimurnikan dan metabolit mungkin perencat penderiaan kuorum (QSI)nya telah dikenal pasti. Pecahan A diasingkan daripada ekstrak S. warneri MG1_8-CFS menggunakan kromatografi lajur gel dan disahkan sebagai pecahan positif dengan keupayaannya untuk menghalang bioluminesen dalam strain
biosensor Vibrio campbellii ATCC BAA-1119 yang menunjukkan 76% perencatan. Selain itu, piridoksina (PN) dikenal pasti sebagai sebatian utama dalam ekstrak S. warneri MG1_8
CFS. Kepekatan perencatan minimum (MIC) PN terhadap E. coli ATCC 25922 ialah 50 mg/mL. PN pada 1/4MIC pembentukan biofilem terturun dan pematuhan sel dalam strain E. coli, seperti yang diperhatikan di bawah mikroskop elektron imbasan (SEM). Selain itu, motiliti dan isyarat AI-2 juga berkurang selepas pendedahan kepada PN pada tahap sub-MIC. Penyelidikan ini menunjukkan bahawa PN boleh merencat pembentukan biofilem dan motiliti dengan mengganggu AI-2 QS, mengesyorkan ia boleh dianggap sebagai agen biokawalan baharu untuk mengurangkan pencemaran bakteria dalam pengeluaran makanan.
Kata kunci: Autoinduser-2; Escherichia coli; pembentukan biofilem; perencat pengesan kuorum; piridoksina
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